Abstract
Abstract:
This study evaluated the effect of Low Level Laser Therapy (LLLT) and Mesenchymal Stem Cells
(MSCs) on bone regeneration.
Background data: Although several studies evaluated the effects of MSCs and LLLT, there is little information
available regarding in vivo application of LLLT in conjunction with MSCs.
Methods: Forty-eight circular bone defects (6 mm in diameter) were prepared in the calvaria of 12 New-
Zealand white rabbits. The defects of each animal were randomly assigned to 4 groups: (C) no treatment;
(L) applying LLLT; (SC) filled with MSCs; (SCL) application of both MSCs and LLLT. LLL was applied on
alternate days at wavelength of 810 nm, power density of 0.2 W/cm2 and a fluency of 4 J/cm2 using a
Gallium–Aluminum–Arsenide (GaAlAs) diode laser. The animals were sacrificed after 3 weeks and then
histological samples were evaluated to determine the amount of new bone formation and the remaining
scaffold and inflammation.
Results: The histological evaluation showed a statistically significant increase in new bone formation of
LLLT group relative to the control and the other two experimental groups (p < 0.05). There was no significant
difference in bone formation of the control group compared to experimental groups filled with
MSCs. Laser irradiation had no significant effect on resorption of the scaffold material. In addition, inflammation
was significantly reduced in LLLT group compared to the control defects and the other two experimental
groups.
Conclusion: Low level laser therapy could be effective in bone regeneration but there is no evidence of a
synergistic effect when applied in conjunction with MSCs.