Abstract
•Gangliosides play crucial roles in adhesion, proliferation, and signalling in neuroblastoma cells.•This approach is helped to improved sensitivity, specificity, and comprehensiveness in ganglioside analysis.•Key challenges include method standardization, sample complexity, and multi-omics integration in trials.•Tackling these hurdles needs cross-disciplinary teamwork and innovation to improve neuroblastoma care.
Gangliosides play important roles in neuroblastoma research through their functions in adhesion, proliferation, and signalling. Researchers have quantified neuroblastoma cells to understand their importance and identify potential diagnostic targets. This study analyses methods for neuroblastoma cell ganglioside quantification. Scientists use techniques including mass spectrometry (MS), nuclear magnetic resonance (NMR) spectroscopy, fluorescence imaging, immunoassays, and advanced approaches like multidimensional chromatography and imaging mass spectrometry. These advances have made ganglioside analysis more sensitive and comprehensive. Researchers perform ganglioside analysis for biomarker discovery, metabolism investigation, and therapeutic agent development. Although progress has been made, challenges remain in standardizing methods, resolving sample complexity, and integrating multi-omics data. Researchers must collaborate across disciplines to overcome these challenges. Ganglioside analysis provides insights into neuroblastoma biology. By leveraging analytical advancements and addressing limitations, researchers can improve pediatric cancer treatment.