Abstract
This study evaluated the antibacterial and anticancer properties of S. mauritianum fruit components
through LC-QTOF-MS/MS metabolomic profiling. The samples were extracted, and the antibacterial
activity was conducted using a standard Resazurin microtiter assay. The minimum inhibitory
concentrations (MICs) of the crude extracts were evaluated against reference pathogenic bacterial
isolates. The anticancer activity of the extracts was tested against U-87 MG glioblastoma and
A549 lung carcinoma cells (ATCC cancer cell lines). The real-time toxicity assay and comprehensive
metabolomic profiling were evaluated for the crude extracts. Results revealed that the ripe fruit
coat exhibited the richest chemical diversity, with 15 unique metabolites, while the unripe fruit had
5. Detailed classification of the identified metabolites showed that alkaloids accounted for 33.3%,
followed by terpenoids (21.2%). The extracts of the fruit components had significant antibacterial
activity against the referenced pathogens of public health importance. Extracts from the ripe fruit coat
demonstrated significant cytotoxicity on U-87 MG glioblastoma cell viability, suggesting potential
anticancer activity, while the effect on A549 lung carcinoma cells showed high viability across all
treatments. The real-time cytotoxicity assays further highlighted the dose-dependent inhibition of
glioblastoma cells by crude extracts from the ripe fruit coat, emphasizing its therapeutic potential.