Abstract
Nanoparticles (Nps) are the basis of nanotechnology, they are very small, with size ranging from 1 to 100 nm and with at least 1 dimension. Nps have broad range of applications, including anti-cancer. Cancer of the breast is amongst the deadliest cancers, especially in women, every year approximately 14 million new cases are reported and about 8 million deaths take place globally. Synthesis of Nps via biological approach has many advabtages over the other methods. Hence, the aim of this study was to synthesize Gold Nps (AuNps) utilizing Sig B and VP Hexane extract as reducing and capping agents, then evaluate the cytotoxicity activity of these, on Breast cancer cells MCF-7. Using Ultraviolet-Visible spectrophotometry, the peaks at 500 nm − 560 nm validated the availability of AuNps. The presence of the Sig B and VP Hexane extract on the exterior of the AuNps was determined utilizing Fourier Transmission Infrared spectroscopy, and average hydrodynamic size was found to be 73.35 and 92.22 d.nm respectively, while zeta charges were -42.7 and -52.8 respectively. Transmission Electron Microscopy provided the actual sizes for these AuNps and they were 10.84 to 57.51 nm and 8.93 to 80.89 nm, respectively. Both Sig B- and VP Hexane−capped AuNps showed cytotoxicity activity agsinst MCF-7 cancer cells in a concentration dependent manner. The MCF-7 cells showed morphological changes upon treatment with the AuNps. These AuNps could serve as anticancer agents for Breast cancer.
M.Sc. (Nanoscience)