Abstract
The purpose of this research is to thoroughly evaluate the effectiveness of Synthesised Gold Nanoparticles in reducing the inflammation and cytotoxicity levels within the bodies of patients diagnosed with chronic inflammatory autoimmune diseases such as Rheumatoid Arthritis and Systemic Lupus. In doing so, the research aims to present a set of robust models to address cytotoxicity and inflammatory issues brought about by these diseases, and which will additionally benefit other chronic and rare medical conditions. The research applies a mixed methods approach, both qualitative and quantitative methods, to carry out the study and makes use of both primary and secondary data collection methods to gather observational, simulation, experimental, and derived data. Comparative analyses and engineering of predictive and logistic regression Monte Carlo models and simulations pertaining to the hypothesis has been performed on MATLAB computational software using the MATLAB and Python programming languages. Transmission Electron Microscopy images have been programmatically created using the SerialEM software and MATLAB toolboxes by simulating the exposure of an electron beam to 15 nanometre bare Gold Nanoparticles for the purpose of gaining a thorough understanding and having a clearer picture of the structure and behaviour of Gold Nanoparticles, as well as to further support the accurate analyses presented in this study, and to arrive at conclusions. 15nm synthesised dendrimer-coated Gold Nanospheres displayed the least cytotoxicity potentials and highest cellular uptake rate at lower concentrations compared to smaller and larger Gold Nanospheres, over a period of 24 hours, and 15nm dendrimer-coated Gold Nanospheres displayed even higher rates of cellular uptake and lower cytotoxicity potentials at higher temperatures, over longer periods of time. Overall, 15nm dendrimer-coated Gold Nanospheres at higher concentrations, as well as longer exposure times proved to be safe and most stable.