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Design and construction of nucleotide probe on an s-scheme heterostructure : experimental and in-silico
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Design and construction of nucleotide probe on an s-scheme heterostructure : experimental and in-silico

Antony Okinyi Onjwaya
Master of Science (MSc), University of Johannesburg
2025
Handle:
https://hdl.handle.net/10210/520069

Abstract

This study investigated the design and construction of a nucleotide probe on a Bi2WO6/NbSe2@Nb4N3Tx MXene S-scheme heterojunction for the photoelectrochemical detection and degradation of efavirenz. The materials were synthesized using a combination of hydrothermal, controlled calcination, and ultrasonication methods. Bi2WO6 was synthesized through hydrothermal methods, while NbSe2 followed a ball milling and calcination approach. Nb4N3Tx MXene was obtained by exfoliating the MAX phase using in-situ prepared HF from a LiF and HCl mixture. The synthesized materials were characterized using X-ray diffraction (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM), energy dispersive X-ray spectrometry (EDS), Raman spectroscopy, Fourier transform infrared spectroscopy (FTIR), UV-visible diffuse reflectance spectroscopy (UV-DRS), and photoluminescence spectroscopy (PL). XRD analysis confirmed the successful preparation of orthorhombic Bi2WO6 phase, hexagonal phases of NbSe2 and Nb4N3Tx, and their respective binary and ternary nanocomposites. While SEM analysis revealed a multicomponent design where a hexagonal-like NbSe2 nanorod and Nb4N3Tx MXene nanosheets formed a support network for reticulated Bi2WO6 micro-flowers. Electrochemical analysis, including cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), chronoamperometry measurements, open circuit potential (OCP), and Mott Schottky analysis, confirmed the formation of an Sscheme heterojunction from n and p-type semiconductors belonging to Bi2WO6 and NbSe2, respectively. This unique architecture established a dual-drive chargetransfer channel, effectively promoting the separation of charge carriers. One path formed a Schottky junction between NbSe2 and Nb4N3Tx MXene, while the other followed an S-scheme transfer mode between Bi2WO6 and NbSe2. To impart high specificity for efavirenz detection, a nucleotide probe was designed using in-silico and molecular docking approaches and functionalized on the photoanode surface using Material Studio, Dmol3 calculation. The docking studies were conducted against various potential pollutants, with the result showing that ...
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