Abstract
M.Sc.
A comprehensive histology-based fish health assessment was implemented at the Roodeplaat Dam (RD), Pretoria, South Africa, to determine the health status of two fresh water fish species, Clarias gariepinus (n = 20) and Oreochromis mossambicus (n = 18), after exposure to pollutants. RD is known to be polluted, being impacted from two sewage treatment plants, industry, housing and agriculture all upstream of the reserve. Excessive nutrient loads, such as orthophosphate, resulted in the dam becoming eutrophic. The quality of water in the RD was a cause for concern, as the fish from the dam are being used as a source of food by the local people.
The histology-based fish health assessment included fish necropsy, calculation of gross body indices and histological assessment. Water quality (chemical and physical) analysis was also carried. Furthermore, target chemical analysis of fish tissue (skeletal muscle) was performed to determine the levels of contaminants in the consumable parts of the fish. These results were compared with existing international guidelines to determine whether the harvested fish were safe for human consumption. All the information obtained was integrated in a qualitative and quantitative scenario-based health risk assessment.