Abstract
Abstract : A nuclear-targeting fluorescent SPIONs-gold core-shell meso-tetrakis(4- hydroxyphenyl)porphyrin conjugate was synthesized, characterized and used for fluorescence imaging and magnetic-targeting in photodynamic therapy against breast cancer cells (MCF-7). In addition, series of gluconic acid capped SPIONs, SPIONs-gold and meso-tetrakis(4-hydroxyphenyl)porphyrin derivatives were synthesized vianew greener approaches and characterized using ultraviolet-visible spectrophotometry (UV-Vis), Fourier Transform infrared spectroscopy (FT-IR), transmission electron microscopy (TEM), high resolution transmission electron microscopy (HRTEM), selected area electron diffraction (SAED), X-ray diffractiometry (XRD), vibrating sample magnetometry (VSM) and energy dispersive X-ray spectroscopy (EDS). After conjugation, the obtained polymeric nanomagnetic porphyrin conjugate was highly soluble in water and exhibited ultraviolet, indigo, blue and red emissions at specific excitation wavelengths varying from UV@254 nm to red@680 nm with high magnetic response to external magnetic field. Furthermore, the conjugate together with some other newly synthesized conjugates displayed high singlet oxygen generation potentials sufficient for the eradication of MCF-7 breast cancer cells in vitro. In addition, the conjugate showed no cytotoxicity against the cancer cells in the dark but became highly toxic to cells after irradiation with light of 673 nm for 14 min 51 s. Moreover, the cells that were exposed to external magnetic field displayed higher phototoxicity than those that were without exposure. Overall, these results indicate that the nano-porphyrin drug system exhibit excellent potential to function as a new promising magnetic-field targeting agent for theranostic photodynamic eradication of cancer diseases.
Ph.D. (Chemistry)