Abstract
D.Phil. (Biochemistry)
This thesis concerns a study of the effect of isonitrosoacetophenone on plant metabolism.
Three different systems were investigated; cultured tobacco and sorghum cells as well as
Arabidopsis thaliana plants, and a metabolomic approach was followed. Unlike most
scientific studies, metabolomics is a discipline which is not driven by a specific hypothesis,
but rather by the obtained data to add scientific insights to the topic under investigation. As
such, the current study lacks a definite overarching hypothesis, but specific objectives were
outlined and answered in each experimental chapter. This thesis is therefore presented as a
compilation of nine chapters in which experimental/research work is described in Chapter 3-
8. It is important to note that each chapter is presented in accordance with the guidelines for
the respective journal in which the corresponding manuscript was published or submitted to.