Abstract
D.Phil.
The determination of several physiologically important molecules
has been carried out using an oxygen electrode and
various oxidase or oxygenase enzymes.
The enzymatic reactions are monitored either by measuring
the initial rates of oxygen uptake from the medium or by
calculating the total amount of oxygen consumed in the reactions.
Because of the use of enzymes, the determinations
are rapid and sensitive and each one is highly specific.
Versatility of analysis can be introduced into the system
by suitable choice of the enzyme. Furthermore, the methods
are simple, and colour or turbidity does not interfere, thus
enabling even whole blood to be used directly as sample
material ...