Abstract
Electrochemical biosensors have provided a new route to biomedical diagnosis and testing. Research to constantly improve biosensor performance and develop new protocols in biosensor design is ongoing. This work contributes to the field of biosensor development by exploring the applicabilities of conductive polymer (CP) and metal nanoparticles (MNPs) based nanocomposites as tools in electrochemical biosensor fabrication. In general, voltammetric and pulsed technique were employed to model the electrochemical reactivities of the CPs- MNPs on working glassy carbon electrodes; while the transmission electron microscopy (TEM), Scanning elelctron microscopy (SEM), UV-vis spectrophotometer (UV), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS) instrumental techniques were used for further characterisation.This thesis focuses on both the integration of functional nanomaterials to improve electrical interfacing between biological system and electronics. Metal nanoparticles based nanocomposite was prepared by using in situ polymerization and composite formation (IPCF) technique and used in the development of an electrochemical sensor for neurotransmitters (NTs) detection with simultaneous reduction of dyes. The MNPs-CPs composite was deposited on the glassy carbon electrode (GCE) by using the drop and dry method. The integration of various nanomaterials is described, in order to understand the effect of different surface modifications and morphologies of various materials for electrochemical sensing of biological analytes. IPCF approach are promising technique to provide key building blocks of nanocomposites for future practical systems, as well as model systems for fundamental research.
A composite architecture of amino acid and gold nanoparticles has been synthesized using a generic route of IPCF. The formation mechanism of the composite has been supported by a model hydrogen atom (H∙≡H+ + e-) transfer (HAT) type of reaction which belongs to the...
Ph.D. (Chemistry)